| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Megasphaera elsdenii | AAGCGCTGGAAGGCATGA | TGCTGGCATCGACCGTAT | 59.65 | 58.78 | 177 | 42 |
100.00%
|
100.00%
|
| Primer 522 | Megasphaera elsdenii | AGCACCAAAGCCGTCGAA | TGGCTTTCGTGCAGTCGA | 59.89 | 59.58 | 277 | 40 |
100.00%
|
100.00%
|
| Primer 523 | Megasphaera elsdenii | ACGTCGATGGCGTCATGA | ACAACTGCTGCATGGCCA | 59.12 | 60.20 | 234 | 40 |
100.00%
|
100.00%
|
| Primer 524 | Megasphaera elsdenii | TGCGGTTCTTCGGCAGAT | CGTCGAAACCGAGGCTCTT | 59.34 | 60.08 | 193 | 40 |
100.00%
|
100.00%
|
| Primer 525 | Megasphaera elsdenii | TGTGCTGCATCGTCTCGA | GGGGAGCAAATTCCTGGCT | 59.04 | 60.00 | 282 | 40 |
100.00%
|
100.00%
|
| Primer 526 | Megasphaera elsdenii | TGTGCTGCATCGTCTCGA | AGGGGAGCAAATTCCTGGC | 59.04 | 60.00 | 283 | 40 |
100.00%
|
100.00%
|
| Primer 527 | Megasphaera elsdenii | TGTGCTGCATCGTCTCGA | GGCAGGGGAGCAAATTCCT | 59.04 | 60.00 | 286 | 40 |
100.00%
|
100.00%
|
| Primer 528 | Megasphaera elsdenii | GTCCTGGAAATGGACCGCA | CGACACACTCCTTCGCCTT | 60.00 | 60.01 | 265 | 40 |
100.00%
|
100.00%
|
| Primer 529 | Megasphaera elsdenii | GTCGAAGGGCAGCATCAGT | CGACACACTCCTTCGCCTT | 60.08 | 60.01 | 289 | 40 |
100.00%
|
100.00%
|
| Primer 530 | Megasphaera elsdenii | ACGCAAATTCCGCATGGC | CCAATGGCTGCCCAAGAGA | 59.82 | 60.00 | 214 | 40 |
100.00%
|
100.00%
|